Cell

Part:BBa_J72138

Designed by: Josh Kittleson   Group: Anderson Lab   (2012-01-20)


JTK160C: MC1061 O16::repA+FRT

JTK160C is a derivative of MC1061 with the repA gene from ColE2 integrated into the O16 locus, allowing it to support replication of plasmid bearing a split ColE2 origin. For more details, see PMID 21787416. The sequence describes the modified region, including local genomic context.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1628
    Illegal XbaI site found at 2787
    Illegal XbaI site found at 3856
    Illegal PstI site found at 137
    Illegal PstI site found at 2758
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1628
    Illegal NheI site found at 1649
    Illegal NheI site found at 1672
    Illegal PstI site found at 137
    Illegal PstI site found at 2758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2743
    Illegal EcoRI site found at 1628
    Illegal BglII site found at 1637
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1628
    Illegal XbaI site found at 2787
    Illegal XbaI site found at 3856
    Illegal PstI site found at 137
    Illegal PstI site found at 2758
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1628
    Illegal XbaI site found at 2787
    Illegal XbaI site found at 3856
    Illegal PstI site found at 137
    Illegal PstI site found at 2758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 34


[edit]
Categories
Parameters
None